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Briefly, urine samples were diluted 100-fold with dilution buffer and then incubated for 1 h at room temperature with polyclonal anti-human L-PTGDS antibody immobilized to the surface of the plate wells.
Urine samples were diluted 1∶15 in molecular biology grade water such that assay values were contained within the measurable range of the standard curve.
Prior to assay, urine samples were diluted between 1∶2 and 1∶12 (depending on the C-peptide level and amount of urine available) with IBL sample diluent (Art.
Urine samples were diluted 10 times before the measurement.
Urine samples were diluted in nitric acid (0.7%).
Throughout the experiment, all the urine samples were diluted by 10 times before concentrations were measured.
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The hydrolyzed urine sample was diluted with 1 mL phosphate buffer (0.1 mol L−1, pH 2.4) [ 7].
For calcium imaging experiments, each urine sample was diluted 100 times with Ringer's solution (in mM: 125 NaCl, 2.5 KCl, 2 CaCl2, 25MgCl2, 25 NaHCO3, 10 HEPES and 10 glucose).
11 A 3 ml portion of the FirstBurst urine sample was diluted with 6 ml of deionised water in a centrifuge tube and then centrifuged at 3000 g for 20 minutes at room temperature.
Of particular note, 10% of 24-hour urine samples were dilute [ 6] and thus, subject to the same problem as the random urine samples in the current study.
Plasma and urine (acidified) samples were diluted 1 1,000 using ultrapure polished water containing 1% v/v HNO3.
More suggestions(15)
bladder samples were diluted
plasma samples were diluted
urine samples were centrifuged
urine samples were provided
urine samples was diluted
urine samples were used
urine samples were measured
urine samples were found
urine samples were treated
urine samples were collected
urine samples were done
urine samples were spiked
urine samples were obtained
urine extracts were diluted
urine samples were prepared
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