Serum samples were analysed for the presence of natural sexual steroids by gas-chromatography-high-resolution mass spectrometry (GC-HRMS), while the presence of synthetic steroids and GCs in urine samples was detected through LC-MS/MS.
After an overnight fast, a pre-test urine sample was collected to detect any endogenous mannitol and the patients ingested 100 ml of the test solution containing 2 g of mannitol, 2 g of xylose, 10 g of lactulose, and 25 g of sucrose in water.
PCR-positive samples were detected in 17 (9.1%) urine samples, their prototypes being as follows: 2/17 (11.2%) M. genitalium, 1/17 (5.9%) M. hominis, 12/17 (70.6%) Ureaplasma urealyticum, 1/17 (5.9%) mixed M. genitalium and M. hominis, and 1/17 (5.9%) M. hominis and U. urealyticum, respectively.
Glucose in blood and urine samples was effectively detected by this strategy.
Glucose in blood and urine samples was effectively detected via this strategy.
Three positive urine samples were equally detected by the two kits and boiling urine samples eliminated 6 false-positive results with use of the Sofia Legionella FIA.
Jung and co-workers (27) developed a liquid chromatography– mass spectrometric method that allows all antihypertensive drugs or their metabolites in a single urine sample to be detected in a qualitative manner.
In addition, the guideline recommends taking a dipslide test or a laboratory test to detect complicated infections if the urine sample is from a male, or to detect antimicrobial resistance.
The data indicate that other constituents that are nonspecific to vomeronasal neurons, such as high concentrations of volatiles and salts in urine samples might be detected to limit the sample access.
Table 4 illustrates the recovery studies of urine samples, and it was detected in the range from 97.1 to 99.3 % with relative standard deviation (RSD) of 1.13 %.
