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Every urine sample was analyzed by two independent technicians.
To determine Hsp72 concentration, the urine sample was analyzed by Western blot analysis and ELISA.
A resting urine sample was analyzed for the presence of albumin by the Royal Hobart Hospital pathology department.
In each series, an internal control urine sample was analyzed, and there was no temporal variation in the measured concentrations of any of the metabolites in the control sample.
The urine sample was analyzed in the Laboratory of the Industrial Toxicology and Occupational Medicine Unit (Université catholique de Louvain, Belgium) without knowledge of their exact provenance in relation to exposure (blind analysis).
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The weight of the collected urine was measured, and three 1 mL aliquots of the urine sample were analyzed in a gamma counter (PerkinElmer, 2480 Wizard).
Blood and urine samples were analyzed using a Dade Behring Dimension RxL chemical analyzer (Siemens, Germany).
The urine samples were analyzed to determine arsenic metabolite concentrations.
Urine samples were analyzed for nitrogen.
Urine samples were analyzed for phosphorus, calcium, and magnesium.
Urine samples were analyzed after 1/50 (v/v) dilution with 5% (v/v) nitric acid.
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