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The shuttle has put secret military satellites into orbit and been a source of scientific work that ranges from the sophisticated monitoring of the effects of long spaceflight on the human body to the "Fun With Urine" experiment that flew on the final Columbia mission and involved purifying urine to make paint.
Just like the fresh urine experiment, 9 out of 12 mice spent more time near the urine marks obtained from control animals.
All thresholds were within 3 standard deviations of clinically normal results, and no subject was excluded from Experiment 1 or Experiment 2. Further analyses of these data suggested that the threshold for the phenyl ethyl alcohol was unrelated to whether a subject could detect the asparagus odor in urine (Experiment 1, r(16) = 0.18, NS; in Experiment 2, r(15) = −0.06, NS).
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In this specific case of infection in the insect_A replica plating strategy was used, contrary to the mouse and urine experiments, because a tetracycline-resistant contaminating flora was present in the insect preventing us to select resolved bacteria on this antibiotic.
By using 3 mL of urine, the experiment began with a single hydrolysis, deproteinisation and extraction step.
FCB carried out and analysed the urine visualisation experiments, TB carried out and analysed the urine-blocking experiments.
Additionally, the urine pH experiments were useful for informing the guidelines for subsequent experiments testing activatable fluorescent agents in response to transient alkalinization.
Urine visualisation experiments demonstrate that notwithstanding the aggressive context of female signal release, male courtship behaviour (seizing, turning) was always preceded by female urine delivery.
For urine pH experiments, SCID mice received 21 mg or 84 mg of sodium bicarbonate p.o. Urine was obtained by applying gentle abdominal pressure against the mouse for 10 sec over plastic film.
Experiments involving aged urine preceded the experiments with fresh urine.
At the end of the experiment, urine was collected and serum was separated for biochemical measurements.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com