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Muscle mass was estimated from 24 h urinary creatinine excretion, assuming that 1 g per day of urinary creatinine excretion represents 20 kg of muscle mass [30].
Complete 24-h urine collections were defined as those where total urinary creatinine was >1000 mg.
Urinary creatinine content (UCC) has been investigated as a promising approach for health states monitoring.
Thus, urinary creatinine levels may be used as an index of standardization.
Furthermore, the concentration of urinary creatinine was measured by the transmitting optical density (O.D).
Urinary creatinine significantly decreased in ischemia-reperfusion group compared to the other groups (P < 0.05).
urinary creatinine.
24-h diuresis urinary creatinine.
Biomarkers were assayed by ultraperformance liquid chromatography-tandem mass spectrometry, normalized by urinary creatinine concentration.
Spot urine protein level was 112.7 mg/dl; urinary creatinine level was 75.5 mg/dl.
Urinary creatinine was measured colorimetrically using a commercially available assay kit (Sigma Aldrich).
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