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Basal respiration in treatments with urea input were 9.4 29.1% higher than that in control.
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This will have important implications for biofuel production where reducing inputs (urea-based fertilizers) is highly desirable for production on nutrient-exhausted land [ 5].
Besides the chemical input as fertilizer, urea is also a natural organic molecule synthesized by most organisms [ 5, 6].
Field experiments using low fertilizer input (20 kg N-urea) and foliar application of both HS from vermicompost and a plant growth-promoting bacteria strain of H. seropedicae (Z67) were carried out [6].
Total endogenous N flows at the duodenum exceeded, by nearly twofold, estimated inputs of urea-N to microbial biomass.
The cumulative inputs of potassium (as K2O), thomas meal fertilizer (as P2O5) and urea-N were fixed for the one-hectare farming input.
As the carp/tilapia combination performed the best in the first experiment, it was tested with different inputs in the second trial, i.e. regular urea fertilization and two different feeding levels.
The initial inputs to stream water were mainly in organic form (as urea) or as ammonia-N.
Critical input variables in the design included waste-frying oil (X1-carbon source), urea (X2-nitrogen source), Na2HPO4/KH2PO4 (X3-phosphates) and inoculum volume (X4), with biomass (Y1) and biosurfactant (Y2) concentrations as response variables.
Northern blots contained 10 μg total RNA (on 2% BPTE agarose gels and 8% PAA, TBE, 8.3 M urea gels), or 2 μg total RNA and 60 μg polyA+ RNA with respect to the input for polyA+ analyses.
All RNA samples were adjusted to the same concentration to homogenize the miR input and 20 micrograms of sRNA were resolved in 17% polyacrylamide gels containing 7 M urea.
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