Sentence examples for urea buffer from inspiring English sources

Cells were harvested and lysed in 8 mol/L urea buffer.

It was denatured and refolded in 8 M urea buffer and purified to 97% purity by passing through a gel filtration column.

Most of the functional ScFv was generated when the inclusion bodies were solubilized in a detergent, air oxidised in the presence of CuSO4 and then denatured in urea buffer in comparison to other protocols.

These were air-dried and dissolved in urea buffer.

To obtain whole cell extract, epidermis was lysed in urea buffer (8M).

Upon centrifugation, protein pellets were resuspended in 100 µl of 0.1 M Tris/1 M urea buffer (pH 8.0).

M-hMPV protein was absent from supernatant and was then extracted from pelleted bacteria by a 6M urea buffer.

RIPA buffer-insoluble pellets were dissolved in urea buffer (7 M urea, 2 M thiourea, 4% CHAPS, 30 mM Tris-HCl, pH 8.5) and sonicated.

Briefly, 900 mg from a frozen tumor was homogenized in 4M guanidine buffer, clarified by centrifugation and equilibrated in 8M urea buffer.

To prevent potential carbamylation of proteins in urea [56], we used freshly made urea buffer and performed digestions at room temperature.

Briefly, about 2×108 cells (or 1×108 bone marrow cells) were lysed in urea buffer, trypsin digested lysate were purified by Sep-pak C18 column.