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Hybrid PET/MR imaging can aid in the characterization and localization of the high uptake zone on PET due to the excellent spatial resolution capabilities of MRI.
We suggest that the increased rooting depth and the decreased DOC concentration in the root uptake zone resulting from progressively thawing soil lead to heavy isotopes becoming more and more available for the larch roots in the course of the vegetative season.
The restricted uptake zone is important for enabling accurate measurements of local connectivity immediately adjacent to the uptake zone and for avoiding tracer spillage into white matter and into adjacent cortical areas.
Figure 1 shows a Nissl-stained section at the level of each of the injection sites, the approximate position of the uptake zone is indicated (see Supplementary Discussion).
Figure 4 a, b show the pattern of label resulting from a small (160 μm diameter uptake zone) simulated injection of an anterograde tracer into this model.
In all cases, the uptake zone was characterized with respect to the retinotopic representation of the area injected, sampling of cortical layers, and possible involvement of white matter.
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However, low spatial resolution of clinical PET machines limits identification of small plaques and localization of high FDG uptake zones.
Regularly spaced roots are most efficient in homogeneous soils because they minimize overlap between root uptake zones (Rubio et al. 2001).
We used retrograde tracers that show high sensitivity and restricted uptake zones, the latter making it possible to verify their restriction to a single cortical area (for a detailed discussion of the relative merits of the tracers used here, see Supplementary material in Markov et al. 2011).
We used 2 fluorescent tracers, fast blue (FsB) and diamidino yellow (DY), that have restricted and well-defined uptake zones that we could confine to cortex subserving central visual space (Bullier et al. 1984; Kennedy and Bullier 1985; Perkel et al. 1986; Conde 1987).
In contrast, the spleen has non-homogenous staining pattern having areas with elevated uptake and smaller zones with lower retention.
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