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Subsequent antigen binding and uptake were measured using flow cytometry.
The DOTA-knottin 3-4A peptide was radiolabeled with 64Cu, and tumor cell binding and uptake were measured using U87MG glioblastoma cells (Figure S3).
The initial rates of sugar uptake were measured using a modification of the method described by Bisson and Fraenkel [ 46].
Associations between the women's characteristics and the CT and FAS uptake were measured using multivariate and multilevel logistic regression analyses.
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LDL uptake was measured using the ImageJ integrated density measurement tool.
Uptake was measured using gamma counting of ex vivo tumours and effect on vasculature by using anti-CD31 microscopy.
Glucose uptake was measured using a fluorescent D-glucose derivative 2- N- 7-Nitrobenz-2-oxa-1,3-diazol-4-yl Amino -2-deoxy-D-glucose (2- N- 7-Nitrobenz-2-oxa-1,3-diazol-4-yl Amino -2-deoxy-D-glucose
Mitochondria were lysed with 50 µl of 1% SDS, and [3H] ADP uptake was measured using a liquid scintillation counter [17].
In all cases the bumetanide-resistant uptake was measured using an identical protocol but with 10 µM bumetanide present during steps 2 and 3. 86Rb+ uptake was terminated by removing the uptake medium and washing cells three times in cold 110 mM MgCl2 using an automatic plate-washer (Multiwash III; TriContinent, Grass Valley, CA).
Thymidine uptake was measured using a liquid scintillation counter.
Glucose uptake was measured using 10 mM [H]2-deoxyglucose [ 19].
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