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Quantification of the tumor uptake was performed using the in-house LabTEP image analysis software.
A comparative evaluation of transfection efficiency, cell viability and cellular DNA uptake was performed using the Lipofectamine™ 2000 (L2K) as a control, and the results show that SF is superior to L2K for MSC transfection.
Subsequent determination of uptake was performed using solutions composed of (in mM) 140 NaCl, 4 KCl, 2 CaCl2, 1 MgCl2, 5 HEPES, pH 7.4 supplemented with 60 µM L-glutamate and 6 nM L-[3H]glutamate (Amersham, Freiburg, Germany) according to the known Km value of EAAT2 for glutamate [19] as well as ceftriaxone at the respective concentration.
The analysis of differences in cell binding and kidney uptake was performed using Student's t-test.
Cell growth was evaluated by MTS assay, intracellular NS uptake was performed using coumarin-6 labelled NS and the amount of intracellular PTX was measured by HPLC.
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In clinical routine, quantification of tracer uptake is performed using the standardised uptake value (SUV).
Statistical tests for alpha diversity among groups of 18F-FDG uptake were performed using the t test and Wilcoxon rank-sum test with the mentioned indices.
Glucose uptake assay was performed using 2-NBDG.
Pyruvate uptake analysis was performed using the following protocol.
The analysis described in section Flux variability vs. uptake rates was performed using the alternative measure of flux variability, Σ, arriving to the same general conclusions (see Additional file 9).
The quantification of the signal enhancement due to Gd-[DTPA] uptake to glioma was performed using Image J software (National Institutes of Health, Bethesda, MD, USA) by calculating the ratio of signal intensity in tumor region to that in normal brain region.
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