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Cellular uptake was evaluated using iron staining method.
DHE uptake was evaluated using confocal florescence microscopy (Nikon Instruments Inc. Tokyo, Japan) and was quantified using ImageJ processing software (FUJIFILM Inc., Minato, Tokyo, Japan).
To investigate if the fluorescent-labeled NC and LNC could be observed by fluorescence microscopy, the nanoparticle uptake was evaluated using a human macrophage cell line.
SWNTs-CpG conjugates were injected into the brain using a single intracranial injection and the uptake was evaluated using flow cytometry and confocal microscopy.
Aortic FDG uptake was evaluated using (1) the mean TBRmax of the whole aorta, (2) the mean TBRmax of the most diseased segment, defined as the hottest slice and the adjacent slices superior and inferior to it, and (3) the proportion of active slices, defined as a slice with a TBR higher than 1.6 [ 25– 27].
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The extent and intensity of all foci of abnormal uptake were evaluated using a 3-point scale (1, low; 2, moderate; 3, high) within each lymphatic area, organ and skeletal region.
Chylomicron remnant-like particles (CRLPs) at three different levels of oxidation (CRLPs, oxidized CRLPs (oxCRLPs) and CRLPs containing the antioxidant probucol (pCRLPs)) and macrophages derived from the human monocyte cell line THP-1 were used as the experimental model, and the mechanisms of uptake were evaluated using specific inhibitors of the processes believed to be involved.
The NS uptake into cells was evaluated using fluorescent Carb-NS and revealed the nanoparticle internalisation.
Quantity uptake of fluoride was evaluated using the Langmuir, Freundlich and Dubinin Radushkevich (DR) models.
The metabolic response was defined by changes in FDG uptake in two liver target lesions, if available, as expressed by the maximum standardised uptake value (SUVmax) and was evaluated using the criteria of the European Organisation for Research and Treatment of Cancer (EORTC).
The [F]FMISO uptake in normal tissue was evaluated using 2D-ROIs on the lung, muscle and a body area 15 mm above the heart.
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