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Transferrin uptake was assayed using flow cytometry.
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Cell cycle arrest and YO-PRO-1 uptake were assayed using flow cytometry, and LC3 redistribution was observed using confocal microscope.
Creatinine was assayed using a spectrophotometric assay.
Uptake was assayed by flow cytometry using a FACScan (BD Biosciences) and quantified on FlowJo software.
Cell uptake was assayed by inductively coupled plasma atomic emission spectroscopy (ICP-AES).
Cellular uptake of the nanovehicle was assayed by confocal laser scanning microscope using rhodamine B (RB) as a fluorescent marker in HeLa cells.
Glucose uptake was measured as described previously by using 2-deoxy-[3H] deoxy-glucose [ 42] and each condition was assayed in triplicate.
When the yeast cells were subjected to D-GalA uptake assays using (H -D-GalA as radio-labeled tracer, we observed substantial D-GalA import over tH -D-GalAound of cells transformed witH -D-GalAty vector, indicasing that GAT-1 was active in the heterologous system.
Aliquots were assayed for activity using the oxygen uptake system.
Cell growth was evaluated by MTS assay, intracellular NS uptake was performed using coumarin-6 labelled NS and the amount of intracellular PTX was measured by HPLC.
Iodine uptake was determined using radioactive iodine.
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