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The efficiency of cell uptake was analyzed using a FACScan flow cytometer (Becton Dickinson, San Jose, CA, USA).

The dye uptake was analyzed using flow cytometry.

Endocytosis of soluble proteins was analyzed by adding either dextran-FITC (2 μg/ml, Sigma-Aldrich) or Lucifer Yellow (2 μg/ml, Sigma-Aldrich) to immature IL-4 or IFNα MoDC for 1 h, after which the uptake was analyzed using flow cytometry (LSRFortessa™).

The cellular uptake of drugs was analyzed using a flow cytometer and FlowJo analysis software.

After 2 h, the EV uptake by macrophages was analyzed using flow cytometry.

Investigation into the role of S611 in the 5HT uptake capacity of SERT was analyzed using the S611A and S611D constructs originally produced, while DD and AA, i.e., the double mutation of T613 and T616, were constructed to investigate the role of the two threonine residues in the proposed mechanism.

Initially, the cellular uptake efficiency of polythiophenes was analyzed using fluorescence microscopy and Tali image-based cytometry.

In the present study, the correlation between 11C-methionine uptake and cell or microvessel density was analyzed using histological specimens obtained by stereotactic biopsy, and an exact local comparison of 11C-methionine PET image and histological specimens was conducted.

To validate the method, the viability of our corticostriatal organotypic slice cultures was analyzed using a standardized protocol for PI uptake.

The percentage of double-positive cells was analyzed using flow cytometry (LSRFortessa™), as a measure of uptake.

ΔΨm was analyzed using JC-1.