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Although regulation of this uptake stage cannot be totally excluded, transformation frequency differences would more likely result from differences of recombination efficiency between the different DNA fragments.
At the uptake stage, it has been previously shown that some bacterial cells may escape mechanical degradation by the pharyngeal grinder (32), with small cells potentially escaping more easily (33).
Embryos were harvested using a fluorescent SV11 stereomicroscope (Zeiss, Germany) to determine morpholino uptake, stage embryos, count somites and perform external phenotypic analysis (Bellairs and Osmond, 1998; Hamburger and Hamilton, 1951).
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BCS uptake among stage I and II patients increased significantly over the study period in Edmonton (P < 0.05), decreased among stage II patients in Central Alberta (P < 0.05) and remained fairly steady in the other regions and in stage III patients over the study period (Additional file 1: Tables S1 1, S1 2 and S1 3).> -wrap-foot> Adjusted for all variables in the table.
A clear pattern that emerged during parameter optimization was that larger d-HSC proportions tend to fit uptake data (stage 2) better.
To measure the metabolic state of developing gametocytes more precisely, C-glucose uptake by Stage III gametocyte-infected RBCs was monitored by C-nuclear magnetic resonance spectroscopy (NMR).
27 This study also analysed whether demographic/other factors relating to the referral were predictors of uptake and stage of exit from the scheme.
The aim of the present study was to examine whether participation in the ERS resulted in increased physical activity and what factors were predictors of uptake and stage of exit from the scheme.
To determine the effect of transport inhibitors on ACF uptake, trophozoite stage parasites were incubated in the absence and presence of different transport inhibitors (furosemide, TP-52, and dantrolene, respectively) for 10 min followed by 100 nM ACF treatment for 5 min as above.
Reason for referral was not found to be a significant predictor of uptake or stage of exit for starters; however those referred by a cardiac rehabilitation nurse (CVD secondary prevention) were more likely to adhere at 12 weeks and those with a prescheme BMI of 30+ kg/m were less likely to complete at 24 weeks.
We found a single false-positive in a patient with suspected peritoneal disease due to disseminated FDG uptake, however staging laparotomy was negative and appearances had been secondary to cholangitis and bile leak at the time of PET-CT.
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