Sentence examples for uptake sequence from inspiring English sources
The 10 bp DNA Uptake Sequence (DUS) GCCGTCTGAA is essential for genetic transformation of N. meningitidis and N. gonorrhoeae.
The obtained PCR products were diluted and amplified with the external primers which also contained the gonoccocal DNA uptake sequence (DUS).
A 10-bp DNA uptake sequence required for efficient DNA transformation of Neisseria [31] was introduced into the 5' end of the kanamycin cassette.
In H. influenzae, the highly redundant USS has the sequence AAGTGCGGT, and this is also the most common uptake sequence in the genomes of H. somni, A. actinomycetemcomitans, P. multocida and M. succiniciproducens [41].
In fact, N. meningitidis is a naturally transformable species and constitutively competent for the uptake of DNA and WGS revealed the presence of about 2000 copies of the 10-bp DNA uptake sequence (DUS) in meningococcal genomes facilitating the incorporation also of foreign DNA.
Plasmid pXen-13 containing the luxCDABE operon was modified to pLKMp by insertion of a Neisseria specific promoter sequence, terminator sequence, kanamycin resistance gene, DNA uptake sequence and two DNA fragments homologous to a non-coding region of the FAM20 genome (Figure 1A and Table 1).
Thus, all commensals appear to have functional DNA uptake sequences.
Among these, Pasteurellaceae and Neisseriaceae species preferentially take up DNA containing specific short sequences, called uptake signal sequences (USS) and DNA uptake sequences (DUS), respectively [3] [9].
DNA Uptake Sequences (DUS) were identified in each genome by looking for the pattern GCCGTCTGAA.
Similar transformation-promoting sequences has been reported as DUS (DNA uptake sequences) or USS (uptake signal sequences) in other Gram-negative bacteria [10], [50] [52].
The current names of pasteurellacean uptake signal sequences (USS) and neisseriacean DNA uptake sequences (DUS) could be misleading, as they do not accurately describe the effect of these sequences on DNA uptake and imply that they represent different genetic elements.
