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The uptake quantification was performed in blind using Oil Immersion 63X objective on more than 200 cells per one single independent experiment (in total of N = 3).
(d) Uptake quantification after 24 hours incubation with primary cultures of hippocampal neurons deriving from FVB Prnp +/+ and FVB Prnp −/− mice.
PET images present a noisy background, making the automatic myocardium extraction and uptake quantification a difficult task.
(a) Uptake quantification after 24 hours incubation with neuroblastoma (N2a) cells that express and that were ablated for the PrPC expression show that 82.1 ± 2.9% of N2a PrP+/+ are able to uptake α-Syn amyloid fibrils in comparison to only 31.8 ± 4.7% of N2a PrP−/− cells.
Again, the highest uptake (quantification level/mm2) was identified in the aortas from ApoE−/− mice (P = 0.026; Fig. 6c).
For tumor [18F]FDG uptake quantification, a manual VOI encompassing the entire tumor was drawn on R10.
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The study found a high congruence between FDG-uptake quantification using the two systems despite the inherent methodological differences between the two systems such as method of attenuation correction, the use of time-of-flight in PET, and the potential interference of the MR signal from PET detectors inside the MR scanner.
Another statistical approach taken to characterize lesion uptake was quantification of the spatial heterogeneity of voxel-based activities in histograms.
Monolayer systems were also used where appropriate for visualisation of photosensitiser uptake and quantification of neurite length.
Most research studies have investigated AV-45 uptake using quantification methods based on target-to-cerebellum standard uptake values (SUVr) [ 5, 8, 16].
SUV (standardised uptake value) quantification on FDG-PET/CT was not found helpful to further clarify the aetiology of muscular swelling, as normal eyes muscles show high physiological FDG uptake and as inflammation could not be ruled out as a cause of increased tracer update in the affected muscles.
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