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In addition, an increased colocalization of E-Cadherin and β-Catenin followed by the cellular uptake of these complexes was apparent upon LecB treatment.
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The uptake of these complex ligands may be facilitated by CXCL16, which may influence this differential compartmentalization [ 9].
The complexes were characterized by dynamic light scattering and cellular uptake of the complexes was studied in a luciferase-based plasmid transfection assay.
Studies of the association and uptake of the complexes demonstrated that cell associations generally increased over time, with the uptake level and the time profile depending on the polymer architecture.
Surface charge of polymer/ODN complexes plays an important role in the uptake of the complexes by cells and their physical stability in blood.
Further, uptake of the complex in Neuro-2a, IMR32 and HepG2 cells analyzed by confocal microscopy and fluorescence activated cell sorting, revealed selective requirement of GM1 for entry.
The cell's uptake of the complex was not inhibited by the presence of 10% fetal bovine serum, and its efficacy for the uptake of SA was the highest when compared with commercial reagents and single-anchored-type synthetic receptors.
Such techniques are not fully applicable to HAMLET and tumor cells, however, due to rapid uptake of the complex and loss of membrane integrity as a result of the cell death process.
This finding suggests that serum adsorption on the particles complex surface could facilitate effective interaction of the particles with cell surface membrane, thereby enhancing cellular uptake of the complex formulation and hence increases gene expression.
The cell- or tissue-specific ligand-receptor interaction contributes to the increased efficacy as a result of enhanced uptake of the complex into tumor cells by receptor-mediated endocytosis.
The function of FAR proteins is unknown but may represent a lipid acquisition system in which released FARs bind to lipids followed by uptake of the complex by a specific receptor mediated process.
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