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The uptake of these complex ligands may be facilitated by CXCL16, which may influence this differential compartmentalization [ 9].
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In addition, an increased colocalization of E-Cadherin and β-Catenin followed by the cellular uptake of these complexes was apparent upon LecB treatment.
Further, uptake of the complex in Neuro-2a, IMR32 and HepG2 cells analyzed by confocal microscopy and fluorescence activated cell sorting, revealed selective requirement of GM1 for entry.
The cell's uptake of the complex was not inhibited by the presence of 10% fetal bovine serum, and its efficacy for the uptake of SA was the highest when compared with commercial reagents and single-anchored-type synthetic receptors.
Such techniques are not fully applicable to HAMLET and tumor cells, however, due to rapid uptake of the complex and loss of membrane integrity as a result of the cell death process.
This finding suggests that serum adsorption on the particles complex surface could facilitate effective interaction of the particles with cell surface membrane, thereby enhancing cellular uptake of the complex formulation and hence increases gene expression.
The cell- or tissue-specific ligand-receptor interaction contributes to the increased efficacy as a result of enhanced uptake of the complex into tumor cells by receptor-mediated endocytosis.
The function of FAR proteins is unknown but may represent a lipid acquisition system in which released FARs bind to lipids followed by uptake of the complex by a specific receptor mediated process.
The extended arene in the functionalized Cp* ligand may play a role in interactions with a target, for example, by intercalation into DNA base pairs, and the hydrophobicity of the arene enhances cellular uptake of the complex.
The complexes were characterized by dynamic light scattering and cellular uptake of the complexes was studied in a luciferase-based plasmid transfection assay.
Studies of the association and uptake of the complexes demonstrated that cell associations generally increased over time, with the uptake level and the time profile depending on the polymer architecture.
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