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Flow cytometry and confocal microscopy results showed significant cellular uptake and internalization of the particles by cells.
Uptake and internalization of all four radioligands was investigated using PC-3 PIP/flu cells (Fig. 2).
The uptake and internalization of drugs intercalated into NDS can be studied with relative ease using in vitro cell models.
Where, the mechanism of uptake and internalization of iron oxide nanoparticles (IONs) into a brain tumour cell were shown [15].
Authors have reported the uptake and internalization of zinc oxide nanoparticles into the human lung MRC5 cells by using TEM investigation.
Fig. 2 Uptake and internalization of all radioligands using a PSMApos PC-3 PIP and b PSMAneg PC-3 flu tumor cells.
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Interaction of LDL with anti-LDL autoantibodies considerably increases the uptake (binding and internalization) of the lipoprotein by arterial cells, which may account for the stimulating effect of antibodies on the LDL-induced accumulation of intracellular cholesterol [ 2].
The cellular uptake and internalization kinetics of the different 68Ga- and 177Lu-labelled PSMA ligands was determined using PSMA-expressing LNCaP cells.
Compared to [68Ga]pentixafor, total cellular uptake and internalization efficiency of [68Ga]NOTA-pentixafor in CXCR4+ Chem-1 cells are 2.6- and 7.9-fold decreased, respectively.
Taken together, our novel method of characterization of cellular uptake and internalization by bladder cancer cells could become invaluable for understanding the role of exosomes on bladder cancer recurrence and progression.
The review also discusses mechanisms of nanoparticle uptake and internalization by cells, obstacles and limitations to the use of this technology, as well as novel methodologies to optimize nanoparticle driven gene expression.
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