Sentence examples for upstream template from inspiring English sources

As mentioned above, a recombination reaction at the DSB end spooled out by fork regression may reestablish a RF through strand invasion at the upstream template (Fig.  2, steps 10 13).

One laser trap holds a polystyrene bead attached to a stalled Pol II molecule, while the other trap holds another bead attached to the upstream DNA template (assisting force geometry; Figure 2A) or to the downstream template (opposing force geometry, not shown).

The footprint length of bound DNA differs among DNA polymerases but generally includes several nucleotides downstream (duplex DNA primer stem) and upstream (ssDNA template) of the nascent base pair (Kunkel and Bebenek 2000), such that at least 9 11 nucleotides are occluded by the enzyme (Rajendran et al. 1998; Swan et al. 2009).

Uncleaved transcription products from the 61 nt-long pGEM-T easy upstream sequence templates were separated in and eluted from polyacrylamide gels.

To design a new set of PCR primers located on the two flanking regions of the gene, the genomic DNA was sequenced by SUGDAT method (sequencing using genomic DNA as a template) upstream or downstream from the respective partial gene sequences.

This generates an HJ-like structure with a recombinogenic double-stranded DNA end homologous to the replication template upstream of the RF.

This software scans within the target range of input sequences and identifies the best TO and EO pair(s) from the library for the purpose of amplifying the target or sequencing the upstream or downstream template.

In other words, the lesion effect was propagated primarily upstream of the template strand and gradually diminished once the adduct came out of the pocket of the polymerase active site.

In silico prediction of MGL RNA secondary structures around chimerization sites of fungal chimeric retrogenes predicts strong hairpin structures immediately upstream of known template switching sites (Additional file 3) that theoretically could slow down reverse transcription.

In contrast, structures of pol β with active site mismatches indicate that the incorrect templating base is positioned upstream of the coding template base pocket creating a pseudoabasic site (i.e., coding potential of the templating pocket is lost).

To accommodate the wrong incoming nucleotide in the closed polymerase conformation, the templating base vacates the coding position with an upstream shift in the template strand.