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Such a variation in accuracy of predictions indicates that we are still far from understanding the regulatory architecture of pre-mRNA 3'-end processing regions lacking the upstream strong PAS site.
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Adam Gordon (University of Washington, USA) presented a new high-throughput sequencing platform, PGRNseq, targeting 84 genes (including 2 kb upstream) with strong associations to drug phenotypes.
Additional files 1, 2, 3 and 4 show the 57 motif groups identified in the 13 sets of upstream regions; strong and weak motif groups, and all motif-sets, motif occurrences and sequence logos are shown.
By contrast, a 2-kb region further upstream gives strong, specific expression in AMsh and PHsh glia, in AMso and PHso socket glia, and in an unidentified pair of neurons in the head.
Also, the ~ 100-bp upstream region exhibited strong constitutive promoter activity by an unknown mechanism(s).
The dynamics of the turbulent flame base, and the fluctuations of its streamwise position, thus appears to be controlled by the intricate coupling between autoignition and the upstream propagation of strong pressure waves sustained by combustion, pertaining to an intermittent detonation-like mechanism.
It's like trying to swim upstream against a strong current.
The v-eQTL is also 28 bp upstream of a strong eQTL signal (rs34387655).
The CMV enhancer and promoter contained a canonical TATA box and a strong upstream activation sequence that together can initiate strong transcription.
1. Upstream work needs strong foundations: UNICEF staff, government officials and partners came back to examples of the work that UNICEF had done historically in Zimbabwe.
This suggested that the upstream region includes strong expression signals that could be used to design a construct to report gene expression in response to Trichoderma-root interactions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com