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Interestingly, we found by screening yeast proteins harboring potential prion-forming domains for additional upstream start sites that Pgd1, a subunit of the RNA polymerase II mediator complex, also possesses an alternative, upstream start site.
Glimmer for example assigns the start site as the most upstream start codon [ 5].
Upstream start codons (uAUG) within the 5′-UTR can inhibit protein translation of an mRNA (Calvo et al. 2009; Yun et al. 2012).
This fragment, however, lies near the 5' region of the gene and there is no upstream start codon within the fragment's open reading frame.
Reporter expression was observed in head and tail nerve cells when gfp was inserted immediately after the most upstream start codon, that for transcripts c/d (WBID Expr9799).
In Drosophila, approximately 40% of transcripts contain upstream start codons (uAUGs) but there is little evidence that these are translated and affect their associated mORF.
Similar(11)
The whales attracted crowds to the banks of the Sacramento River as they meandered upstream starting about May 13.
Upstream regions were defined as 1 kb upstream starting from the translational start site or the transcriptional start sire if a 5′-UTR was annotated.
Most fkh-7 ESTs correspond to transcript b starting from exon 2. There is a small exon 1 far upstream starting transcript a and a small transcript c from the end of the gene.
"The more upstream you start, the more total control," he said.
"When you go upstream, you start seeing needs that don't make a whole lot of sense," said Hindman.
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