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Here, fish from the upstream (reference) site sometimes showed higher concentrations in comparison to the exposure site.
The results indicated significant increases in soluble and total protein contents in fish muscles being exposed to the potential pollutants from the sewage and industrial effluents as compared to the respective values obtained for the fish sampled from the upstream reference site.
Conversely, significant reduction in carbohydrates, total lipids, cholesterol and RNA contents of the pollution exposed fish meat as compared to the respective values of fish muscle from the upstream reference site was evident that the fish health was under strong negative pressure due to the pollutants loads.
Study sites within streams were divided into 1 km upstream (reference) and 1 km downstream (treatment) reaches.
Within each stream, upstream reference and downstream treatment reaches were sampled 1 year before, during, and 1 year after the addition of SCA.
Ten leaf packs were placed at each riffle sampling unit and removed at days 15 (n = 5) and 30 (n = 5) in upstream reference and downstream treatment reaches.
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Land cover changes and channel planform evolution in the restored segment were compared with a downstream non-restored (control) segment and to an upstream unregulated (reference) segment before (2011) and shortly after (2014) the restoration implementation.
The full-scale data has been processed in 12 min non-overlapping record blocks of cube surface tap pressure together with the reference upstream approach flow measured at cube height.
Hence, there must be other reasons, such as pollution with, e.g., hydrophobic contaminants by diffuse sources (e.g., roads, traffic, and agricultural land use) or point sources upstream of the reference site.
Contigs which are upstream of the reference point are assigned negative distances, whereas contigs downstream are assigned positive ones.
In the re-sequencing panel, we performed bi-directional sequencing of 6311 bp per individual, encompassing the most of the SLC2A4 gene and ∼1 kb upstream the gene (Reference sequence: chromosome 17, positions 7124832-7131142 of the NCBI human genome build 36.3).
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