Exact(4)
Other studies have profiled the expression of some proteins in mOGCTs for descriptive and mechanistic purposes, such as for the estrogen receptors 1 and 2 and their coregulators SNRPN upstream reading frame protein (248), GDNF family receptor α-1 (249), and cold shock domain protein A and Y-box-binding protein 2 (250).
89 The genes makorin ring finger protein 3 (MKRnecdinecdiNDNNDN), melanoma-associated antigen-like 2 (MandL2) and SNRPN upstream reading frame (SNURF-SNRPN) are expressed only from the paternal chromosome where their promoter regions are unmethylated.
To find support for this hypothesis, we further analyze whether the length changes caused by complex ASEs and their flanking exons can offset the frame-shifting effects of each other and retain the upstream reading frame.
Only the form containing exons 13, 2, 3 and 4 was amplified (Fig. 2D), consistent with the mouse MOR-1K form (43). Again, consistent with the mouse MOR-1K, translation of these new isoforms seems to be initiated at the first AUG in exon 2, as all upstream reading frames code for short peptides.
Similar(56)
The offset was counted as the distance from the start of the upstream read to the start of the downstream read.
For the march round 3 line, the upstream reads were half of the location-collapsed reads with no barcode (NN) from the initial library lane and the downstream dataset was an equal number of location-collapsed reads with a TT barcode from the lane marched three times.
Briefly, RUD is the ratio between the density of downstream reads and the density of upstream reads (see Methods).
We used the relative usage of downstream poly(A) site (RUD) score, which is (density of downstream reads)/(density of upstream reads).
The relative usage of downstream polyA site (RUD) score was calculated, which is the ratio between the density of downstream reads and the density of upstream reads.
We used RNA-seq to identify distinct 5' ends for many transcripts, characterized by a set of reads with a common 5' end and the absence of upstream reads (GEO accession #GSE26633).
In drawing the distribution of sense and antisense mapped reads around TSSs, we eliminated the furthest-upstream reads composing the transcript-enriched regions because these reads were utilized for the adjustment of the genomic location of TSSs that resulted in the intentional overrepresentation of read enrichment at the TSSs.
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