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Of interest, the organization of the first replicon repFIIA-repFIC, located upstream of the transfer region, is more similar to the replicon identified on pAPEC-O1-ColBM [19] than to the homologue on pAPEC-O2-ColV [15] (Fig. 4), which suggests that ColV plasmids are not necessarily from the same backbone.
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We examined the sequence pattern of regions upstream of the transferred ribosomal genes in the recipient genome.
By itself, this discovery did not identify the mechanism by which the ABCA1 protein facilitates transfer of cholesterol to HDL, but good evidence suggests that it acts upstream of actual transfer of cholesterol from the cell.
This observation suggests that the partially unwrapped histones contact the upstream DNA forming a (tension sensitive) loop that allows their transfer upstream of the elongation complexes.
cDNAs for rat and human Y1 receptors, and the human Y2 receptor, were each PCR amplified to remove start and stop codons, and inserted upstream of the fluorescent protein fragments, before transfer to the vector pCMV FLAG (Stratagene).
Catalase is a heme-containing protein (Klotz 2003), and a cluster of transferred genes upstream of the M. prima's catalase gene (Theba_0063 Theba_0068) could be involved in heme biosynthesis (Supplementary table S2).
Particle Image Velocimetry (PIV) was included to support the heat transfer measurements, suggesting that peak heat transfer occurred 0.12 nozzle diameters upstream of the jet reattachment point along the liner.
The alr4641 promoter and its adjacent DNA were cloned upstream of the gfp reporter gene in reporter vector, pAM1956, and transferred into Anabaena PCC7120 An4641promm).
The position of the transferred gene is also of interest, as it is immediately upstream of the class II FBA in genomes where both are present.
Remove the cover of the transfer switch.
The amount of the transfer fee.
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