Sentence examples for upstream of the mutation from inspiring English sources

The R176Q mutation affects a residue just two amino acids upstream of the mutation (E178G) reported by Nicolino et al. 9 (Fig. 1).

In addition, we found that transcription initiated upstream of the mutation cassette apparently interferes with the ability of I-SceI to cleave at its recognition site.

Briefly, DNA is first amplified by PCR and then linear amplification with base extension reaction which is designed to anneal to the region upstream of the mutation site.

The 5' arm of all OCPs annealed to target upstream of the mutation and was designed to have a Tm of approximately 70°C.

Nonsense mutations in the last exon of a gene are not thought to cause nonsense-mediated decay (NMD) as an exon/exon junction upstream of the mutation is required to bind NMD initiation factors.

Sense and antisense oligonucleotide primers (Table S1 of the Supporting Information) encoding ∼20 bases downstream and upstream of the mutation were used for site-directed mutagenesis and paired with primers upstream and downstream of two restriction enzyme sites, ClaI and XbaI.

Two of the 19 mutations sequenced in the unc-52 study carried a second site mutation less than 400 bp upstream of the primary mutation.

This analysis also yields an additional SNP that falls within the area of PCR amplification, affecting SSA1266 bp upstream of the R57 mutation and leading to a D22G missense mutation, which further supports a determinant role for SSA1 in the observed phenotype.

For the couples who were heterozygous for the β-globin gene mutation at IVS1-5, AS-PCR was carried out to detect SNPs located at the upstream region of the mutation.

Sequence analyses of PCR products suggested that a C/T polymorphism found upstream of these mutations caused the preferential PCR amplification of the mutated alleles.

It is worth noting that when qRT PCR was performed using primers upstream of the Δ3 4 mutationtandon (and hence detect both the WT and mutant Δ3 4 MECP2 transcripts), transcripts were detected from Δ3 4-hiPS #20 (Supplementary Material, Fig. S8a).