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To this end, 1064 bp upstream of the mouse Wnt11 transcription start site was cloned into a luciferase reporter vector.
Interestingly, however, there is an apparent transcript in the mouse X chromosome that is on the minus strand that starts approximately 100 bp upstream of the mouse Fmr1 gene.
The reporter construct used in this assay was derived by inserting ∼1 kb of the sequence upstream of the mouse VEGFA transcription start site, in front of the luciferase gene.
The VEGFA promoter region contains one putative XBP-1-p binding site, the ACGT core [19], [20], 972 bp upstream of the mouse VEGFA start site, raising the possibility that XBP-1-p binds to VEGFA promoter.
To determine if Neurog3 was able to directly activate Math6 gene expression, we cloned a 1.8 Kb genomic fragment upstream of the mouse Math6 translational start site and used it to drive expression of luciferase in pancreatic duct cells.
That PPARγ binds to an enhancer located ∼8.8 kb upstream of the mouse resistin gene transcriptional start site and this confers adipogenic expression of mouse resistin has recently been reported [23].
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To test this idea, we derived a reporter construct by cloning 1 kb upstream sequences of the mouse VEGFA transcription start site upstream of the luciferase gene.
In order to delineate the 5' upstream regions of the mouse and human NXNL1 and NXNL2 genes, we determined the position of their putative transcriptional start sites (TSSs) by rapid amplification of cDNA ends (RACE), using RNA purified from mouse and human retinas.
Specifically, in an upstream promoter of the mouse Hoxa9 gene, the study localized several CpG-rich clusters that were associated with MLL1 [ 54].
Briefly, the regulatory sensor sequence consists of two parts: a) a 484-bp fragment from the upstream region of the mouse Cyp1a1 gene that contains four DREs, and b) a 1,330-bp 1,330-bp fragmentt ofrome mouse mammary tumor virus partofer lacking glucocortheoid-responsive enhancers.
Moreover, a small sequence of 198 bp approx. 90 Mb upstream of the mentioned mouse sequence Chr. 4 position contig.
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upstream of the spacecraft
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