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If several HSPs within the same hit fit these criteria, the more upstream match was chosen.
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These "regulons" can be defined either based on upstream matches to a consensus binding motif or based on the results of a ChIP-chip experiment.
T-profiler is a computational tool that uses the t-test to score changes in the average activity of predefined groups of genes based on Gene Ontology categorization, upstream matches to a consensus transcription factor binding motif, or KEGG pathway [19].
Without our SNP-tolerance feature, this SNP would preclude a consecutive 14-nt stretch of upstream matches needed by GSNAP for genomic localization.
Most mapped promoters had upstream matches to the RpoE2 promoter consensus sequence, thus the corresponding genes are likely direct targets of RpoE2.
The k a are adjusted to reflect the relative importance of the parameters; for example it is more important that the difference in melting temperatures be close to zero than it is that the distance to the upstream primer match the distance to the downstream primer.
Concentration of Cu at upstream sites matches with the category of unpolluted status; the values however were found to be higher than WHO norms at downstream sites.
In addition, when enrichment of upstream consensus matches for individual genes is used to define putative cis-regulatory targets at a false discovery rate of 5% (see Methods), a significant number of genes is found only for E2F (Figure 1C).
In this study we scored differential expression at the level of gene sets to infer changes in the activity of transcription factors from the mRNA expression levels of the genes predicted to be under their control, based either on upstream sequence matches to cis-regulatory elements (motif-based regulons) or on occupancy by a specific transcription factor (ChIP-based regulons).
* indicates only the upstream gene matches.
The spectra obtained for the PQS from the var gene upstream B regions match expectations for G-quadruplex structures.
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