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Upstream leaders like Ethiopia's prime minister caution that Nile water use is "not a zero-sum game," but in Egypt's Delta that's exactly how millions of farmers view it.
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To test whether the upstream leader region of fhlA facilitates the interaction with OxyS and Hfq as previously described for the rpoS-DsrA system [23], the leader sequence of fhlA was extended from the previously characterized position −53 relative to the start codon, to −136 and −220 respectively.
However, a few bacterial genes lack such upstream untranslated leaders, as first shown for the cI repressor of bacteriophage λ [33], then the tetR gene of transposon Tn1721 [34].
Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical.
We inserted the 5' leaders upstream of the Photinus luciferase gene.
For RNA transfections, in vitro transcripts were generated using intron-free CAT-FLAG reporter genes containing the T7 promoter sequence upstream of the 5' leaders.
We hypothesized that some features of the 5′ leaders upstream of uORFs may be important for resistance.
So, we hypothesized that some features of 5' leaders upstream of uORFs may be important for the regulation.
However, the upstream sequence from Leader 1, Ex1 (1.084 kb), generated a P∶R ratio comparable to that observed from Ex2 indicating that Ex1 contains an IRES.
In S. marcescens Db11, the thrABC operon presents upstream its respective leader sequence and peptide (thrL).
Also here translation occurs from a leaderless transcript, because the TSS is at only one nucleotide upstream of the leader peptide's start codon.
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