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Thus, 5′ end of TSS region was curated to a position where +TAP profile of 1 nt upstream genomic position is no higher than 20% of the position.
Based on the 5′ upstream genomic sequences of SH4, a primer pair (psh4F-xba: 5′-AGAGGCTCTAGATTCGATTCCC-3′ and psh4R-bgl: 5′-GCAGAGAGATCTGACATGCTCG-3′) was designed (the underlined letters indicated XbaI/BglII restriction sites) to facilitate directional cloning of the promoter.
The mir-200c hairpin coding sequence and approximately 300 bp of upstream genomic sequence is CpG rich.
pCZ483 includes both genes of the tba-1 operon (tba-1 and drsh-1) and 2 kb of upstream genomic sequence.
A partial deletion of the PTE and neighboring upstream genomic sequences by imprecise excision of the P element also results in a similar loss of Abd-B expression in embryos.
Further molecular analysis of a mutant generated by imprecise excision of the P element reveals that a partial deletion of the PTE and neighboring upstream genomic sequences results in a similar loss of enhancer-directed Abd-B expression.
Similar(10)
In cases in which no UTR information was available, for the respective transcript 100 bp of the respective 3'-downstream and 5'-upstream genomic sequence were added.
Size selection by gel electrophoresis is used to enrich the resulting PCR product for molecules that contain most of an Alu insertion as well as 50-300 bp of upstream flanking genomic sequence.
A stop codon occurs only upstream of the genomic island, even if that means overlapping substantially with an adjacent genomic island or ssrA itself.
The tag alignment position's upstream and downstream genomic sequences (200 bp each) were extracted from the genome assembly using homemade Perl scripts.
PCR amplification using primers homologous to upstream and downstream genomic regions of the NAP1L3 gRNA target site were used to generate DNA fragments that were sub-cloned for sequencing of in total 10 individual clones.
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