Sentence examples for upstream genome from inspiring English sources

These regions possess the same upstream genome border located near an epsP synthase, and the downstream border near a tonB-dependent receptor, except for the xop10 element, where the downstream gene is located close to a methyltransferase.

A recent study of the regulatory region of one mouse OR gene revealed that a small transgene containing merely ~400 bp upstream genome sequence is sufficient to drive mutual exclusive expression of this transgene [ 21].

We assumed that the surrounding nucleotide composition of these genes may be the genuine trigger, the upstream genome structure of these genes are more stable and avoid the insertion of non coding DNAs or other genes which leads to the shorter interval between adjacent gene; however this assumption requires further validation.

The plasmid pIDSB21, for deletion of xer, was constructed by sequentially cloning PCR products of the xer upstream (genome coordinates: 319885 319141) and downstream (318443 317686) flanking regions into the KpnI– NcoI and BamHI– NotI sites, respectively, of pET2268 (Szabó et al, 2007).

Implementation of marker-assisted breeding, including genome wide selection and upstream genomics studies such as genome-wide association studies, requires large numbers of robust markers that are widely distributed across the genome.

We first examined the effect of upstream parameters: genome features (size and repeated elements prevalence) and AFLP parameters (number of selective bases, GC content) on homoplasy.

The 5′-capped GFP-PRRSV mRNA) was first transcripted by T7 DNA polymerase in vitro with the transcription kit using Acl I-linearized FL12-GFP plasmid as a template (there is a T7 promoter upstream PRRSV genome in FL12 plasmid).

The other (referred to as insertion 2) is located at the 3′-UTR of the gene coding for a hypothetical protein of unknown function which is conserved and syntenic in all publicly available Leishmania genomes (upstream gene localisers LmjF.29.2290; LmxM.08_29.2290BPK_282220.1, LmxM.08_29.2290; Ltand9.2440 and LbrM.29.2260).

Exact copies of this pair, with 100% nucleic acid identity including the flanking inverted repeats, were found in two other places in the genome: upstream of the bamB homolog (Gmet_1788-Gmet_1789), and upstream of the oah homolog (Gmet_3281-Gmet_3282).

To isolate additional upstream sequence further genome walking was performed using the PvuII genomic DNA library giving a new 1,721 bp PCR product.

In the hospital biosafety level 2 laboratory, MERS-CoV screening was performed by real-time reverse-transcription (rRT) PCR on respiratory samples by checking for the upstream E protein genome (Roche Modular Dx Coronavirus) and infection confirmation by detecting the open reading frame 1a genome (MERS-CoV kit from TIB Molbiol) [14].