Sentence examples for upstream frame from inspiring English sources
yongonense strains, and not in other examined strains (Additional file 2).> As shown for other IS elements of the IS 21 family [ 28], the M. yongonense-specific IS elements also exhibit two consecutive open reading frames: a long upstream frame, designated istA (1,578-bp 1,578-bpshorter downstreandframe, deshorterdownstream3-bp).
To find support for this hypothesis, we further analyze whether the length changes caused by complex ASEs and their flanking exons can offset the frame-shifting effects of each other and retain the upstream reading frame.
In addition to this, the ATG upstream in frame characteristic is highly relevant since the best results were obtained by the combination of this with other features.
An ATG upstream in frame can be explained by the ribosome scanning model, which scans from region 5' to region 3' until it finds the first ATG which contains a translation context.
89 The genes makorin ring finger protein 3 (MKRnecdinecdiNDNNDN), melanoma-associated antigen-like 2 (MandL2) and SNRPN upstream reading frame (SNURF-SNRPN) are expressed only from the paternal chromosome where their promoter regions are unmethylated.
The PCR products were cloned into pAG3-AP containing an upstream in-frame AP cassette.
Subsequent Nco I digestion was used to insert NS5A1-31 immediately upstream, in-frame to rat MVP cDNA previously cloned into pFastBac-1 vector using standard molecular biology techniques.
Start codon positions are determined by examination of ATG motifs present upstream, in-frame or within 30 bp downstream of the beginning of the aligned reference protein.
If no upstream, in-frame ATG codons were found, we retained the most complete, computer-generated gene model for further analysis and annotation.
Actually, if the more complete sequence had been cloned in the original work, the Authors would certainly have registered the upstream in-frame AUG as the start codon.
The methionine encoded by the most upstream in-frame ATG (nt 466 of NaRpoTp) aligns to amino acid residue 125 of Arabidopsis RpoTp, and the amino terminus derived from this position displayed neither plastid nor mitochondrial targeting properties (see below).
