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Recently, Lo and colleagues were not able to detect LigB among L. interrogans serovar Lai samples shifted from environmental to human body temperature (37°C), demonstrating that temperature upshift alone may be insufficient for induction of expression of Lig proteins [88].
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But you can downshift all the way to second, come to a stop, accelerate in first gear, and the car will only upshift as far as redline in second gear, leaving the job of upshifting beyond that point to you.
This correlates with an upshift that is seen with the kinase domain alone, and which is not seen with a T162A mutant.
Intriguingly, an upshift was seen with the kinase domain fragment alone upon serum starvation that was not present with the T162A mutant, suggesting that serum starvation is also accompanied by phosphorylation at this residue.
In a straight line there's nothing to do but watch for the 6,000 rpm redline and upshift, as 75 mph is possible in 2nd gear alone.
we observed the appearance of a new band, corresponding to the miR-509-3p/PNA2 complex, which was upshifted relative to the bands of the two components alone.
Gears can be changed only in numerical order; it is not possible to upshift from first to third or downshift from fourth to second.
" 'Cracklin' Rose.' Especially when I upshift.
Alone, alone.
You'll need quick hands to grab an upshift during the split second between maximum power and fuel cutoff.
When the driver decides it is time for an upshift (using the floor shifter or steering-wheel paddles), the first clutch releases and the second one takes hold.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com