Sentence examples for upper strand of the from inspiring English sources

The upper strand of the sodium bisulfite treated DNA was amplified using specially designed PCR primers [47] to yield a 439 bp band from the p1 distal enhancer (positions -5104 to -4666 of EF165349).

The cDNA was again purified with Agencourt AMPure XP, followed by treatment with Shrimp Alkaline Phosphatase (Affymetrics) and USER NEBB) to restrict the upper strand of the 3′ linker.

When the result of roulette in GMB is like this picture, the rotatable fifth base (see arrow in this figure) of the upper strand of DNA will change A to C. By reference to the mutation table (Fig. 3), this DNA change will cause the shift of "size of the wings": the front wing will be smaller and the back wing will be bigger Fig. 3 Mutation table.

As a competitor of SAF-1 binding, some binding reactions contained 50 pmol of a canonical SAF-binding double-stranded oligonucleotide having an upper strand sequence of 5′-CCCTTCCTCTCCACCC-3′.

The primer is the same sequence as the mismatched part of the upper strand, and this prevents initiation of priming from the vectorette until the complementary strand has been synthesised by priming from within the retroviral insertion.

As a nonspecific oligonucleotide competitor, a random sequence of oligonucleotide with an upper strand sequence of 5′-TCGAACTGAACTTGAG-3′ was used.

We further examined the occurrence pattern of sequencing errors, as shown in Fig.  1E; the average sequencing error rates at each base in the 50 control amplicons are shown in a bar graph, where the bars in the upper or lower direction show the sequence error rates at the base position on the upper or lower strand of the amplicons, respectively.

Taken together our results suggest that the upper strand or bottom strand cytosines (5'-GCAG-3'/3'-CGTC-5') were not the sites of methylation by HP0593 MTase.

The primer has the same sequence as the upper strand and, as with vectorette PCR, cannot anneal until the complementary strand has been synthesised.

Orientation of the motif is indicated on the right (+, upper strand; −, lower strand).

A 0 (0 < A 0 < 1) is the initial frequency of any particular oligonucleotide sequence (which can also be a mononucleotide) in <span class="lh">the upper strand.