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The upper phases from the second ATPS were subjected to protein content and protease activity analysis.
The upper phases from three extractions were recovered and dried on a rotatory evaporator.
The three different upper phases obtained were combined and concentrated to dryness in a speedVac.
The upper phases was extracted a second time by the addition of 1.8 mL of fresh, pre-equilibrated neutral lower phase.
The extraction was repeated with 1 mL ice-cold water and 2 mL of hexane/chloroform 4/1 (v/v), the upper phases were combined and dried under a stream of nitrogen.
Then, the upper phases were pooled in a 10 mL tube, dried under a nitrogen stream in a Nitrogen Evaporator (Organomation, USA) and the residues were weighed and stored at −80°C until analysis.
Similar(50)
The upper phase is the polymer solution and the lower phase is the pure ionic liquid.
Quadratic models were predicted for LAB yield in the upper phase.
Nevertheless, it is enriched in the ionic liquid-containing upper phase of the ATPS.
But, it is also enriched in the ionic liquid containing upper phase of the ATPS.
This system is known as an upper phase microemulsion, Winsor Type II.
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