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Solutions were purified by ultrafiltration (Amicon Ultra 100-kDa filters; Merck) and collected upper phase of the filtration cassette.
After a waiting period of 30 and 300 s of sedimentation time, samples of the upper phase of the vial were taken and investigated by dielectric- and fourier transform infrared spectroscopy (FTIR) to determine the stability of the suspension.
The upper phase of the solvent system in the solvent separator was used as both the PLE solvent and the HSCCC stationary phase, followed by elution with the lower phase of the corresponding solvent system to separate the common ginsenosides.
In both ionic liquid-based systems, complete isolation with simultaneous concentration of the polysaccharide in the upper phase of the system is obtained, and 80% of the initial disaccharide is retained in the lower phase.
Finally, the upper phase of the solvent system of n-hexane n-butanol methanol water (4.2:1.0:1.2:4.5, v:v:v:v) was used as both the PLE solven-hexane n-butanol methanol watertracted at 125 °C), an-hexane n-butanol methanol watered with the corresponding lower phase of the solvent system to separate the low polar compounds.
At the first stage, the upper phase of the solvent system of ethyl acetate n-butanol water (1.1:1.0:2.1, v:v:v) wacetate n-butanol water solvent and HSCCC stationary phase, and the target compounds were eluted with the corresponding lower phase of the solvent system to separate the polar common compounds.
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After a 10 min centrifugation (1000x g, room temperature, without break), the mixture separated into an upper phase of plasma, followed by the white peripheral blood mononuclear cell (PBMC) layer, the separation gel ficoll and erythrocytes in the bottom of a 50 ml tube.
Upper panel shows cluster formation by the upper phase of filtration (>100 kDa MWCO) and lower panel shows cluster formation by the passed-through fraction (<100 kDa MWCO).
The upper phase of heptane was removed and larvae washed with 100% methanol three times.
The upper phase of both 2.0 mL tubes was transferred into a 15 mL tube and 4 mL of RLT buffer and 3 mL of absolute ethanol were added and mixed.
The upper phase of both 2.0 mL tubes was transferred into a 15 mL tube, and 4 mL of RLT buffer as well as 3 mL of absolute ethanol was added and mixed.
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