Sentence examples for upper membrane from inspiring English sources

When the upper membrane was located in the bioreactor, less membrane fouling was observed.

(a) After PNGase F deglycosylation cell lysates of N2a cells treated with different α-Syn prep were separated by SDS-PAGE and PrP was immunoblotted with two Abs that recognize C-terminal part (W226 upper membrane), and N-terminal of the prion protein (SAF34 lower membrane), showing increased levels of α-cleavage product, C1 fragment.

A few options to control the growth of nanotubes inside the membrane channels or on the upper membrane surface were considered and successfully demonstrated.

Non-migratory cells on the upper membrane surface were removed with a cotton swab, and nuclei were stained with 0.5 µg/mL DNA intercalant Hoechst #33258.

Cells were incubated for 48 hours in a humidified 5% CO2/37°C incubator after which non-migrated/invaded cells were removed from the upper membrane and membranes were fixed and stained with 0.2% crystal violet.

The same experiment was also performed using a transwell co-culture system in which Hospicells were cultured in the bottom chamber and OVCAR3 cells were cultured in the upper membrane.

Some sample wells were stained at the completion of the incubation period to ensure that cell adhesion on the upper membranes was uniform under all conditions.

At 24 h later, the upper membranes were wiped with cotton swabs and any cells that had migrated to the bottom side of the membranes were fixed in ice-cold methanol.

Upper membranes were incubated with monoclonal anti-Hsp72 antibody.

GFP expression was not detected in non-transgenic plants or in vector controls (upper blot membrane).

Fluorescence recordings were initially made at 22 °C from the upper plasma membrane of unstimulated 293TR Y1sfGFP cells (Fig. 2).