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For the CDS pooled from the moderate and low expression categories, we found enrichment of certain DNA/RNA and protein synthesis genes, primarily from the upper expression levels in the moderate class.
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The high expression group in each plot (blue) accounts for the upper quartile of expression levels for a particular transcript and the low expression group (red) the remaining 75%%.
Hence, we set our upper limit of mean expression levels at 5 fold to work within that range.
(A ) Upper panel shows Smc expression levels of strains with mutant smc alleles (BSG1002, 1007, 1008, 1045, 1046, 1047 and 1074) determined by immunoblotting using anti-Smc antibodies.
We analyzed the prognostic value according to the median, upper and lower quartile expression levels of p21 in the 20-year follow-up for OS (353 patients) and DMFS (761 patients).
Despite this, one of the transcripts, XLOC_000764, was in the upper 95th percentile of overall expression levels; that is, its expression level was higher than 95%% of detected transcripts, suggesting that it plays a physiologically relevant role.
Still, of the 23 PTP genes detected among the upper 25% by expression level, 12 match for both detection methods.
Upper panel shows expression level of AtLYK5 in Col-0 and Atlyk5-2 mutant plants, lower panel shows expression of AtCERK1 as a control.
In comparison, most Hsp60 and Hsp70 genes showed high expression levels in the upper stem (internode 2 and 3).
Most Hsp60 and Hsp70 genes showed high expression levels in the upper stem (internode 2 and 3), indicating their putative roles in the primary stem growth of Populus.
Similarly, the samples in the upper left branch have elevated PR expression levels, low to medium ER, Bcl-2 and Bag-1 expression levels, and low Her2/neu expression levels.
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Justyna Jupowicz-Kozak
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