A flow of neutralized dried oil is dosed continuously with bleaching clay prior to entering the upper compartment of the bleacher.
For the cell invasion assay, the polycarbonate membranes of the upper compartment of the chambers were pre-coated with a matrix gel.
The polycarbonate membranes (8-µm pore size) on the bottom of the upper compartment of the Transwell chamber were coated with 1% human fibronectin (R&D systems 1918-FN, USA).
The polycarbonate membranes (8-µm pore size) on the bottom of the upper compartment of the Transwells were coated with 1% human fibronectin (R&D systems 1918-FN, USA).
The upper compartment of the chimney-piece is composed of separate pieces of the same diversified material, and the frieze of the upper order also consists of coloured marble in the centre.
Cells were then applied to the upper compartment of the invasion chamber.
Similar amounts of cleaved CXCL12 were also detected in the upper compartment of the transwell.
One day later, the cells were exposed to the air-liquid interface by removing the culture medium in the upper compartment of the insert.
The cell suspensions were seeded to the upper compartment of the Transwell chamber at the cell density of 1×105 in 100 µl serum free medium.
Cells were detached, washed in DMEM containing 0.1% BSA, resuspended in the same medium at a concentration of 5×105/ml, and added to the upper compartment of the chamber.
After a 24-h preincubation, 0.45 µCi/well of [14C]-oleic acid (specific activity of 55 mCi/mmol; GE Healthcare, Piscataway, NJ) was added in the upper compartment of the Transwell for another 24-h period.
