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Distinct chlorophyll lost and a sharp decrease of chlorophyll fluorescence ratio from 0.78 to 0.15 were observed in N. benthamiana leaves transiently expressing litchi SGR, while no obvious degreening was noticed upon transient expression of litchi PPH.
Simulation results illustrate the efficiency and effectiveness of the proposed approach and its impact upon transient behaviour of the microgrid.
Growth reduction of DLD-1 cells upon transient miR-145 transfection, which implies that miR-145 possesses a tumor-suppressor function in vitro has already been reported [36].
However, cell attachment was substantially increased upon transient expression of GFP-myosin IIA.
Nkx3.1 promoter activity and protein level were reduced upon transient expression of ERG in LNCaP cells (Fig. 4G-H).
Upon transient transfection of this vector (pB45Neo-promLUC) into HeLa cells, infection with HSV-1 induced luciferase expression (Fig. 2a).
To this end, we analyzed the phosphorylation state of Lck and Fyn upon transient RNAi of LAT expression (Figure 3A).
These findings indicate that, upon transient induction of RNase L, lipid accumulation was increased in cells cultured in conditions permissive for muscle differentiation.
The resulting physiological localization upon transient transfection into CHO-K1 cells is shown in Fig. 1C (Keq = nuclear concentration/cytoplasmic concentration = 6.5±1.7 in the observed cells).
In addition, the average area of a focal contact was significantly increased upon transient expression of GFP-myosin IIA in the spreading COS-7 cells (Fig. 7D).
These experiments were carried out upon transient overexpression of WT CD95, N118Q, N136Q and N118Q/N136Q in HeLa cells (Figure S3E, F, G).
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