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Studies upon these samples were approved and handled in accordance with the Institutional Review Board of Institute of Biochemistry and Cell Biochemistry, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
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Upon arrival these samples were stored in a standard freezer.
Upon treating these samples with SA beads, the 271 bp bands produced from samples which had utilized the Alien biotin probes were captured.
Because in schizonts and gametocytes before activation the PfGAP50 is part of the actin-myosin motor complex driven invasion machinery (glideosome) associated to the multi-protein complex called inner membrane complex (IMC), the cell membranes were permeabilized by saponin washing upon preparation of these samples.
Upon hydrous pyrolysis of these samples the early released H2S formed secondary pyrite, which was isotopically 21‰ lighter than the bulk organic S, indicating that the system was not in equilibrium [16].
The expression pattern of various apoptosis-related genes was unchanged in these samples upon incubation in 5 or 11 mmol/l glucose (Fig. 2b, c).
Upon subsequent analysis of these samples with LC-FT-MS/MS, 761, 826, and 881 proteins were quantified in WT, SAA012, and SAW041, respectively.
In these samples CD19hiCD38+ samples upon exclusions of cells expressing CD3, CD14, IgD or CD20 were gated onto IgG+ cells.
Our results showed that the pairwise variation values changed depending upon the samples analyzed.
These estimates depend upon the sample size and the smallest, middle and greatest values of the test data.
This method depends upon the sample condition, processing and temperature throughout the process (should be 4 °C).
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