Candidates 28 and 29 were found as a cluster, and upon further inspection of the flanking region, two additional C/D snoRNAs were identified in this cluster.
This result corresponds to earlier observations [e.g. [ 44]] suggesting that the degree of enhancement depends upon the exon sequences flanking the intron; additionally that the sequences surrounding the introns do influence efficiency of intron splicing.
As populations around the world encroach upon the flanks of nearby volcanoes, an increasing number of people find themselves living at risk from volcanic hazards.
Previous reports have concluded that DNA accommodates unpaired nucleobases in both intra- and extrahelical environments (60), the orientations being dependent both upon the identity of the unpaired nucleobase and upon the DNA sequence flanking the site (61).
Upon sequencing of the DNA flanking the Hermes integration site, the clone was found to contain the transposable element inserted within the SPBC13E7.08c (leo1 + ) open reading frame 507 nucleotides from the start codon.
The primers were designed based upon the amino acids flanking the active site cysteine and asparagine residues that are conserved in the eukaryotic cysteine proteases.
The required number of plants per BC cycle depends upon the distance of the flanking markers from the target gene, recombination rate in the chromosomal region, and desired level of homozygosity in the selected plants (Table 3 and 4).
Studies conducted with other ISWI-containing complexes, such as the yeast ISW2, have shown that the affinity of nucleosome binding is dependent upon the length of the flanking DNA with a minimum of 20 bp required for stable binding.
It is likely that the percentage of replaced allele to starting allele containing BACs is at least somewhat dependent upon the size of the flanking sequences used in the allele replacement vector, but construction of allele replacement vectors with exceptionally large flanking sequences is not always practical.
As the SNPs were derived from cDNA sequences, the occurrence of intron/exon border in the flanking sequences upon which the oligos are later designed, may cause significant genotyping failure [ 51].
