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Moreover, the substrates of OWmISG15 identified upon Tandem Affinity Purification followed by LC-MS/MS identification largely outnumbered these of HuISG15 itself.
In addition, the GP-derivatised sterols give a characteristic fragmentation pattern upon tandem MS (MSn) analysis.
Each tag adds the same molecular mass, but upon tandem mass spectrometry (MS/MS) analysis, a unique reporter ion is detected for each condition.
All charge states for modified peptides were included in the analysis, and the abundance of isobaric co-eluting modified forms were deconvoluted based upon tandem MS data as previously described [ 46].
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In the crystal lattice, two molecules form a tandem dimer upon binding to adjacent sites on DNA.
During our daily laboratory work, we hit upon a discordant short tandem repeat (STR) genotype at the D2S1338 locus between Identifiler Plus®16 system kit and Power Plex®21 system kit.
Based upon this analysis, one tandem SNP target (rs432114 rs365433) which showed a third (fetal) peak on subject FDT0604 was not predicted to be highly informative.
The sequences within the two blocks of tandem repeats upon chromosome 4 were found to be closely related suggesting these have all arisen from one ancestral sequence which was present at this location and has subsequently been tandemly duplicated.
Nonetheless, all the M. africanum West African-1 strains in the Ghana strain collection had RD711 deleted and, as part of another study [ 24], could be further subdivided phylogenetically based upon differences in mycobacterial tandem repeats numbers.
Previous studies have determined that the ability of c-FLIP to drive NF-κB activation is dependent upon the presence of two tandem DED motifs [8].
Next, we wanted to test whether paternal allele-specific expression of AK029869 depended upon the presence of the tandem DNA repeats in the ICR, as is the case for Rasgrf1.
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