A bioassay using Culex pipiens larvae revealed that the mosquitocidal activity was virtually lost upon replacement of loop2.
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An insignificant effect on purity descriptors and percentage yield of TP upon replacement of acetone with methanol during predicted vs observed correlation studies (being similar polarity index of 5.1) pointed towards overwhelming influence of solvent polarity.
Of course, the economic cost of the hybrid includes the price of the battery, both as original equipment and upon replacement, and this price is not included in the mileage per gallon calculation.
Moreover, upon replacement of Mg++ with Mn++, IAV Pol displayed increased polymerase activity, but with significantly reduced processivity, and misincorporation was slightly elevated in the presence of Mn++.
Upon replacement of CHX by the RNA-polymerase II inhibitor actinomycin D (ActD) immediate early mRNAs, which have been transcribed in presence of CHX, become selectively translated.
DDM1 and MET1 are required to maintain a silent chromatin state associated with CG methylation [40], [41], [43], which is not immediately re-established upon replacement of functional DDM1 or MET1 [65], [66], [66].
Upon replacement of the three proline residues in human ubiquitin with 4-fluoroproline isomers we could confirm that it is possible to stabilize globular proteins by applying the pre-organization principle.
Indeed, direct binding of DBB to H-Ras could be evidenced by fluorescence detection of the gels, taking advantage of the fluorogenic properties of this compound upon replacement of both bromines [25] (Fig. 7C).
The hypothesis that R224 is the sensor was supported by the loss of pHo-sensitivity after its neutralization, and by the modulation of the pHo-sensitivity upon replacement with amino acids of different pKa values, or alterations in its environment.
Upon replacement of all Pro residues in EGFP by either (4R -FPro or (4R -FPro we were nor only able to control protein folding, but also to dissect the contributions of various factors to the folding of a complex protein molecule.
