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The formation of blebs results from a localized rupture of the interaction between actin filaments and the plasma membrane [26], [27], leading first to membrane expansion, then rapidly followed by blebs retraction upon reassembly of the actin cortex.
After 30 min at pH 7.0, the far-UV CD spectrum was almost identical to that of the native CtxB5 at pH 7.0, indicating significant recovery of the native secondary structure upon reassembly (Fig. 3C).
For K. aerogenes urease, the full complex is also reformed upon reassembly.
As a result, the half-zippered SNARE state was typically short-lived (<0.2 ms) upon reassembly of the SNARE complex at the low forces favoring NTD association, because the trapping force opposing CTD zippering remained small immediately after NTD was zippered.
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Upon mathematical reassembly of the halves the registered force imbalance is assigned to be the lift force.
Therefore, this eliminates the possibility that envelope reassembly upon exit from mitosis could contribute to alterations in nuclear envelope structure.
The disassembly of the contractile apparatus upon isolation and a reassembly from pre-myofibrils after attachment to cell culture substrates seem to be decisive for this environmentally controlled adaptation (Du et al., 2003; Sanger et al., 2005).
Reassembly is assessed by comparing the reassembled complex to the native complex.
The protein subunits are constantly undergoing a process of reversible disassembly and reassembly during the cell cycle and upon a variety of stimuli particularly those related to oxidative stress, apoptosis-inducing or exposure to toxins [30].
The reason why the heat shock alone partially represses PHO5 transcription is unclear, but it is accompanied by partial reassembly of the chromatin in an Spt6-independent manner upon the heat shock (see later).
In order to directly detect nucleic acid polymers, we have designed biosensors comprising sequence-specific DNA binding proteins tethered to split-reporter proteins, which generate signal upon binding a predetermined nucleic acid target, in an approach termed SEquence-Enabled Reassembly (SEER).
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