Sentence examples for upon permeabilization with from inspiring English sources

Since these structures are lost upon permeabilization with 0.2% Triton X-100, we utilized 0.1% saponin to preserve them, as previously described12.

In contrast, the p-EGFR levels were only moderately reduced upon permeabilization with digitonin compared with saponin extraction, suggesting that the majority of p-EGFR faced the cytosolic surface of endosomes and was not within ILVs.

Some individual cells expressing tdTA (as seen by fluorescence) did not display any Ca2+ activity (luminescence) upon ATP stimulation, but a strong signal was imaged upon their permeabilization with saponin at the end of the experiment, which brings millimolar external Ca2+ in contact with the probe.

To this aim, we performed immunofluorescence colocalization analysis between MAPK15 and endogenous autophagic vesicle markers, upon permeabilization of cells with digitonin in order to eliminate the background signal due to their cytoplasmatic forms.

Only upon permeabilization of the cells with toluene was significant conversion of xylitol observed, indicating that cytoplasmic localized, wild-type AldO is unable to react with xylitol probably because xylitol is unable to pass the inner membrane.

When we performed a similar staining comparison in THP-1 and Jurkat cell lines, we observed relatively smaller increases in BST-2 expression upon permeabilization of the cells (Fig. 3C and D).

Upon permeabilization, 5 × 10 PBMCs and 2 × 10 tissue lymphocytes were re-suspended in 100 μl of PBS and incubated for 30 min with anti-pSTAT1 A-2 -PE anti-pSTAT1 A-2 -PET3(B-7)-PE anti-pSTAT1 A-2 -PET-bet(4B10)-PE anti-pSTAT1 A-2 -PEz Biotechnology, Santibodyz, Canti-pSTAT3 B-7 -PE

Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining.

Intracellular staining for Foxp3 PE-Cy7 (FJK.165, eBioscience) was performed after fixation with Foxp3 Fixation Buffer and permeabilization with Foxp3 Permeabilization Buffer (eBioscience).

This was followed by permeabilization with 0.1% saponin and intracellular staining with anti-BST-2, and subsequently stained with secondary antibody (anti-rabbit-Alexa Fluor 488).

Vero cells were fixed with 4% PFA (Electron Microscopy Sciences) for 10 min at room temperature before permeabilization with 0.2% Triton X-100 (Sigma) for 5 min at room temperature.