Your English writing platform
Discover LudwigExact(2)
The observations show a rather special structural properties of the DNA binding domain, in which the recognition helix h3 adopts a 310 helix and the center-to-center separation between recognition helices h3 and h3' is reduced from 34.9 Å to 30.4 Å upon inducer binding.
In all TetR members structure-known to date the recognition helix h3 takes α-helix type conformation and the corresponding center-to-center distance is otherwise increased upon inducer binding, e.g. from about 34 Å to 39 Å, 41 Å and 52 Å for TetR [5], [11], QacR [6], [18], [19] and EthR [8], respectively.
Similar(58)
In addition, the main apparent conformational change of the DNA binding domain induced by effector binding is the variability of the separation between DNA binding motifs (h2 h3 and h2' h3'), which is shortened from 34.9 Å to 30.1 Å upon the inducer binding, while increased from about 34 Å to 39 50 Å in other TetR members identified to date [5], [6], [8], [11], [18], [19].
Next, we studied cell growth upon the inducers' addition.
Upon addition of the inducer (cumate), USP10 can be induced to higher levels in both myoblasts and myotubes.
The authors placed one of the protein components of the viral replicase (1a) under the control of the XVE chemically inducible promoter in such a way that recombinant viral amplicons could be induced upon addition of the inducer (β-estradiol).
Inoculation of the engineered bacteria into a microalgal culture in the absence of sources of C and N other than N2 and CO2 from the air, resulted in a strong proliferation of microalgae that was suppressed upon addition of the inducer.
Glycerol does not induce cellulase gene expression in T. reesei, but does not prevent induction of cellulase gene expression upon addition of an inducer [ 37].
The threshold value of stress required to activate apoptosis inducer depends upon the regulatory mechanism that controls (or contributes to) its switch-like activation.
The iCasp9 suicide gene can induce apoptosis upon specific binding with a chemical inducer of dimerization (CID) AP20187 (Fig. 3).
Becskei et al. (2001) [26] finally proved that in yeast even a less complex network based on the tet-system can result in a bimodal expression pattern upon gradual increase of the inducer tetracycline.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com