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For the first time, we demonstrate that ND3 is not the only subunit to become exposed upon deactivation of mitochondrial complex I.
Complete localization of T-3-BP-AuNPs at 12 h might also be due to the opening of the VDACs upon deactivation of HK2 by 3-BP.
ND3, ND1 & 39 kDa subunits become exposed upon deactivation of complex I. Supercomplex composition is not affected by mitochondrial complex I conformation.
Another main finding of this study is that ND1, a core membrane subunit, is also affected by structural rearrangements upon deactivation of complex I.
The relocation of the hydrophilic part of ND3 upon deactivation of the mammalian enzyme could lead to a change in the quinone chamber, affecting interaction of the quinone headgroup with its binding site [ 1].
We find that, similar to its regulation in osteoblasts, RUNX2 expression in MDA-MB-231 breast adenocarcinoma cells is enhanced upon growth factor deprivation, as well as upon deactivation of the mitogen-dependent MEK-Erk pathway or EGFR signaling.
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Exposure of Cys-39 of the ND3 subunit upon deactivation is one of the main conformational changes identified in complex I to date.
Upon the deactivation of the ABC transporters by NaN3, there is no ABC efflux pumps-associated alkane efflux during alkane import.
This finding indicates that, upon deactivation, the position of Cys changes and it can be cross-linked to one of the lysine residues of the 39 kDa subunit in the vicinity.
The fact that the A/D transition has a high activation energy [ 10] favours the idea of significant conformational changes upon deactivation.
Upon intracellular Ca2+ sequestration, deactivation of nNOS would proceed in reverse order.
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