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Both parameters depended linearly on the steady state voltage upon current injection, and values were interpolated to give the value at −100 mV to allow comparison.
In contrast to FS interneurons, non-FS interneurons showed marked adaptation of AP firing upon current injection (Table 4).
Neurons rarely spontaneously fire action potentials under such conditions (Fig 7A, control = 0pA), because all synaptic inputs were blocked, but fire upon current injection (Fig 7A).
We recorded mEPSCs in electrophysiologically defined fast-spiking interneurons (predominantly PV-expressing interneurons), identified by their high-frequency action potential firing upon current injection and characteristic action potential shape, of TrkB.T1-broad mice and control littermates (Fig. 3 A).
Here, we distinguished 2 main interneuron classes on the basis of their AP firing pattern and passive properties: (1) Fast-spiking (FS) interneurons that were characterized by their sustained high-frequency firing upon current injection, and (2) low-threshold spiking (LTS) interneurons that possessed low rheobase values (Fig. 8 A, B and Table 4).
Similar(55)
Neurons were considered GABAergic based on the following electrophysiological criteria: continuous fast firing without spike frequency accommodation upon depolarizing current injection, fast spontaneous firing frequency >10 Hz and an action potential half-width ≤1.0 ms.
Cells were characterized based on the pattern of action potentials generated upon somatic current injection and also filled with biocytin, allowing for post hoc identification of their morphological characteristics.
In the latter case only action potentials generated during the first 10 ms upon onset of the current injection which just crossed the action potential threshold were considered.
As NPS is a modulator of some aspects of fear and extinction, we examined oscillatory activity on the LA in the presence of NPS. Figure 6A shows a representative example of subthreshold membrane potential oscillations upon membrane depolarization by a steady current injection (85 pA, duration 8 s) in an LA projection neuron.
Here, current injection triggered a sustained, step-like jump in membrane potential upon which small amplitude spikelets (mean frequency = 51.49 ± 6.14 Hz) occurred.
Using somatic current injection, thus, avoids the potential confounds introduced by short-term plasticity upon repetitive stimulation of the same fibers.
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