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Artemia nauplii were the most adequate food source during first feeding of the pacu, and they produced bigger fish upon completion of the experiment.
Upon completion of the experiment, one preparation was gently turned ventral side up, and the silicone probe inserted in order to match the previous dorsal side up configuration; the activity patterns obtained in the dorsal side up preparation were verified in the inversed set-up.
Participants were compensated with $20 upon completion of the experiment.
Upon completion of the experiment, the final electrode position was marked by passing a current through each electrode, thus creating a small electrolytic lesion.
Upon completion of the experiment, participants received small gifts.
Participants were paid £7 upon completion of the experiment.
Tumours were measured every 2 days or upon completion of the experiment.
Upon completion of the experiment, participants were thanked for their participation and were fully debriefed.
Upon completion of the experiment, the survival animals were euthanized by overdosed intravenous injections of pentobarbital sodium (Tianyi, Xian, China).
Upon completion of the experiment at 96 hours, the fish were snapped frozen in liquid nitrogen and stored at -80°C for subsequent analysis.
Upon completion of the experiment the animals were deeply anesthetized with chloral hydrate (350 mg/kg, intraperitoneally) and decapitated; the brains were removed, immediately frozen and kept at − 80 °C.
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